Validation Data Gallery
Product Information
Nano-Secondary ® anti rabbit IgG / anti human IgG recombinant VHH, Alkaline Phosphatase is an anti-human IgG and anti-rabbit IgG secondary Nanobody (VHH). It is a fusion of a VHH that binds to human IgG and rabbit IgG with high affinity & specificity, and one AP molecule. Note that AP will dimerize. This product uses recombinant, animal-production free Alkaline-Phosphatase, expressed as fusion-protein with the VHH in E.Coli.
Description | Alkaline Phosphatase allows for a broader range enzymatic detection compared to HRP. The VHH format delivers excellent specificity, with no cross-reactivity against serum proteins from other species. |
Applications | ELISA |
Host/Type | Alpaca |
Reactivity | Rabbit IgG / Human IgG |
Class | VHH |
Form | Liquid |
Conjugate | Alkaline-Phosphatase (recombinantly produced) |
Molecular weight | 61.5 kDa (monomer); 123 kDa (dimer) |
Immunogen | Purified rabbit IgG |
Affinity | KD = 0.2 nM |
RRID | / |
Storage Buffer | 20 mM Tris pH 8.0, 250 mM NaCl, 1 mM MgCl2, 0.1 mM ZnSO4, 50% glycerol |
Storage condition | -20°C / -80°C |
Shipping | Blue ice |
Size | 1ml |