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Validation Data Gallery

  • HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antibody (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1), 1:500.

    HEK-293 and Hela cell lysates were subjected to SDS-PAGE followed by fluorescent western blot analysis with rabbit anti-GAPDH antibody (10494-1-AP) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1), 1:500.

  • Immunofluorescence analysis of A431 cells stained with rabbit anti-Lamin B1 antibody (12987-1-AP, red) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1), 1:500. Nuclei were stained with DAPI. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells stained with rabbit anti-Lamin B1 antibody (12987-1-AP, red) and Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1), 1:500. Nuclei were stained with DAPI. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Immunofluorescence analysis of A431 cells co-stained with rabbit anti-Lamin B1 antibody (12987-1-AP, red) and mouse anti-B23 antibody (60096-1-Ig, cyan). Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1, 1:500) and Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006) were used for detection. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of A431 cells co-stained with rabbit anti-Lamin B1 antibody (12987-1-AP, red) and mouse anti-B23 antibody (60096-1-Ig, cyan). Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, CoraLite® Plus 555 (srb2GCL555-1, 1:500) and Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006) were used for detection. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

ChromoTek Nano-Secondary® anti-rabbit IgG, recombinant VHH CoraLite® Plus 555 [CTK0119, CTK0120]

Nano-Secondary® anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.
Cat No. srb2GCL555-1
引用文献(1)

Applications

WB, IF

Conjugate

CoraLite® Plus 555

Host

Alpaca

Reactivity

Rabbit IgG

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Formats:  CoraLite® Plus 555
规格价格库存

Concentration: 1 mg/mL

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Product Information

Nano-Secondary® anti-rabbit IgG, recombinant VHH, is a rabbit IgG-specific antibody. This secondary antibody product consists of nanobodies that binds to the Fc-domain of rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-rabbit IgG, recombinant VHH reagent uses a novel class of rabbit IgG-specific antibodies. This secondary antibody product consists of nanobodies that bind to rabbit IgG with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, see details below.
ApplicationsWB, IF
HostAlpaca
Species ReactivityReactivity: Rabbit IgG
No cross-reactivity: mouse, rat and human IgGs.
Target/SpecificityRabbit IgG
Physical StateLiquid
ConjugateCoraLite® Plus 555
Suggested DilutionImmunofluorescence 1:500
Western blot: 1:500
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to mouse, rat or human IgGs
ImmunogenPurified rabbit IgG
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0119 (VHH0708), CTK0120 (VHH0953)
Affinity (KD)CTK0119: KD = 0.2 nM, CTK120: KD = <10 pM
RRIDAB_3094640
Excitation / EmissionExcitation max: 554 nm, Emission max: 570 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Discov Oncol

Peptide antibody tools to dissect specific functions of eIF4A paralogs in cancer.

Authors - Shobhit Srivastava
View Article
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