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CoraLite® Plus 488-conjugated NXT1 Monoclonal antibody

NXT1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C10A2

Cat No : CL488-67680

Print datasheet

Synonyms

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

验证数据展示

  • FC experiment of HeLa using CL488-67680

    FC experiment of HeLa using CL488-67680

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NXT1 (CL488-67680, Clone:2C10A2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67680-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67680 targets NXT1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen NXT1 fusion protein Ag30039 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name NTF2-like export factor 1
Synonyms MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15
Calculated Molecular Weight 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC000759
Gene Symbol NXT1
Gene ID (NCBI) 29107
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKK6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NXT1 antibody CL488-67680Download protocol
Standard Protocols
Click here to view our Standard Protocols