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NXN Monoclonal antibody

NXN Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C6A4

Cat No : 66603-1-Ig

Print datasheet

Synonyms

NRX, nucleoredoxin, NXN, TRG 4

验证数据展示

  • IF Staining of HEK-293 using 66603-1-Ig

    IF Staining of HEK-293 using 66603-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NXN antibody (66603-1-Ig, Clone: 2C6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human kidney using 66603-1-Ig

    IHC staining of human kidney using 66603-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66603-1-Ig

    IHC staining of human kidney using 66603-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66603-1-Ig

    WB analysis using 66603-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66603-1-Ig (NXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66603-1-Ig targets NXN in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse , Rat
Immunogen NXN fusion protein Ag9248 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nucleoredoxin
Synonyms NRX, nucleoredoxin, NXN, TRG 4
Calculated Molecular Weight 435 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009327
Gene Symbol NXN
Gene ID (NCBI) 64359
RRIDAB_2881963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6DKJ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

实验方案

Product Specific Protocols
WB protocol for NXN antibody 66603-1-IgDownload protocol
IHC protocol for NXN antibody 66603-1-IgDownload protocol
IF protocol for NXN antibody 66603-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols