验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66603-1-Ig targets NXN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse , Rat |
免疫原 | NXN fusion protein Ag9248 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | nucleoredoxin |
别名 | NRX, nucleoredoxin, NXN, TRG 4 |
计算分子量 | 435 aa, 48 kDa |
观测分子量 | 48 kDa |
GenBank蛋白编号 | BC009327 |
基因名称 | NXN |
Gene ID (NCBI) | 64359 |
RRID | AB_2881963 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q6DKJ4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
实验方案
Product Specific Protocols | |
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WB protocol for NXN antibody 66603-1-Ig | Download protocol |
IHC protocol for NXN antibody 66603-1-Ig | Download protocol |
IF protocol for NXN antibody 66603-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |