验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13275-1-AP targets NXF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3998 Product name: Recombinant human NXF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC031616 Sequence: MSLPSGHTTGHTDQVVQRRARCWDIYQRRFSSRSEPVNPGMHSSSHQQQDGDAAMHGAHMDSPVRYTPYTISPYNRKGSFRKQDQTHVNMEREQKPPERRMEGNMPDGTLGSWFKITVPFGIKYNEKWLLNLIQNECSVPFVPVEFHYENMHASFFVENASIAYALKNVSGKIWDEDNEKISIFVNPAGIPHFVHRELKSEKVEQIKLAMNQQCDVSQEALDIQRLPFYPDMVNRDTKMASNPRKCMAASLDVHEENIPTVMSAGEMDKWKGIEPGEKCADRSPVCTTFSDTSSNINSILELF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear RNA export factor 3 |
| 别名 | Nuclear RNA export factor 3, TAP like protein 3, TAPL 3, TAPL3, TAPL-3 |
| 计算分子量 | 531 aa, 60 kDa |
| GenBank蛋白编号 | BC031616 |
| 基因名称 | NXF3 |
| Gene ID (NCBI) | 56000 |
| RRID | AB_2877933 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H4D5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NXF3 (nuclear RNA export factor 3) belongs to the NXF family of RNA transport proteins, predominantly expressed in human testis cells. Unlike canonical NXF1, it bears truncated functional domains and lacks typical RNA-binding capacity. It mediates nucleocytoplasmic shuttling of snoRNAs and regulates TGF-β3 mRNA trafficking independent of bulk mRNA export. Recent studies confirm its oncogenic role in gastric cancer, where elevated NXF3 accelerates tumor proliferation, invasion and predicts poor patient survival. It acts as a prognostic biomarker and a potential therapeutic target for gastrointestinal malignancies. (PMID: 29021253)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NXF3 antibody 13275-1-AP | Download protocol |
| WB protocol for NXF3 antibody 13275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Nuclear translocation of CDK5RAP3 regulated by NXF3 promotes the progression of gastric cancer | ||
Sci Transl Med A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins |
