验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10328-1-AP targets NXF1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 |
CatNo: Ag0413 Product name: Recombinant human NXF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 45-349 aa of BC004904 Sequence: SGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear RNA export factor 1 |
| 别名 | MEX67, mRNA export factor TAP, TAP, Tip associated protein, Tip associating protein |
| 计算分子量 | 70 kDa |
| 观测分子量 | 66-70 kDa |
| GenBank蛋白编号 | BC004904 |
| 基因名称 | NXF1 |
| Gene ID (NCBI) | 10482 |
| RRID | AB_2236500 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBU9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NXF1 antibody 10328-1-AP | Download protocol |
| IP protocol for NXF1 antibody 10328-1-AP | Download protocol |
| WB protocol for NXF1 antibody 10328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.
| ||
Nucleic Acids Res Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy. | ||
PLoS Genet Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.
| ||
Mol Cell Biol Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.
| ||
Chromosoma XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1. |
