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NWD1 Polyclonal antibody

NWD1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25025-1-AP

Print datasheet

Synonyms

NWD1, NWD1 Ens

验证数据展示

  • WB analysis of SH-SY5Y using 25025-1-AP

    WB analysis of SH-SY5Y using 25025-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25025-1-AP

    IF Staining of HepG2 using 25025-1-AP

    Immunofluorescent analysis of HepG2 cells using 25025-1-AP (NWD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells, mouse brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25025-1-AP is NWD1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen NWD1 fusion protein Ag21754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NACHT and WD repeat domain containing 1
Synonyms NWD1, NWD1 Ens
Calculated Molecular Weight 1564 aa, 175 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC117698
Gene Symbol NWD1
Gene ID (NCBI) 284434
RRIDAB_2879855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ149M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeat. The molecular weight of NWD1 was detected by western blot. The result shows that the molecular weight of NWD1 is 77 kDa., but NWD1 is a 174 kDa protein according to UniProt,. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.

实验方案

Product Specific Protocols
WB protocol for NWD1 antibody 25025-1-APDownload protocol
IF protocol for NWD1 antibody 25025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EBioMedicine

Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.

Authors - Qin Yang
View Article