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验证数据展示

  • WB analysis of SH-SY5Y using 25025-1-AP

    WB analysis of SH-SY5Y using 25025-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25025-1-AP

    IF Staining of HepG2 using 25025-1-AP

    Immunofluorescent analysis of HepG2 cells using 25025-1-AP (NWD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NWD1 Polyclonal antibody

NWD1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25025-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

NWD1 Ens, NACHT domain- and WD repeat-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25025-1-AP targets NWD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 NWD1 fusion protein Ag21754 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NACHT and WD repeat domain containing 1
别名 NWD1 Ens, NACHT domain- and WD repeat-containing protein 1
计算分子量 1564 aa, 175 kDa
观测分子量 77 kDa
GenBank蛋白编号BC117698
基因名称 NWD1
Gene ID (NCBI) 284434
RRIDAB_2879855
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ149M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeat. The molecular weight of NWD1 was detected by western blot. The result shows that the molecular weight of NWD1 is 77 kDa., but NWD1 is a 174 kDa protein according to UniProt,. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.

实验方案

Product Specific Protocols
WB protocol for NWD1 antibody 25025-1-APDownload protocol
IF protocol for NWD1 antibody 25025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EBioMedicine

Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.

Authors - Qin Yang
View Article