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验证数据展示

  • WB analysis using 16970-1-AP

    WB analysis using 16970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16970-1-AP

    IF Staining of HepG2 using 16970-1-AP

    Immunofluorescent analysis of HepG2 cells using 16970-1-AP (NVL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

NVL Polyclonal antibody

NVL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16970-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

nuclear VCP like, Nuclear VCP like protein, NVL, NVLp

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, mouse lung tissue, HeLa cells, mouse kidney tissue, rat thymus tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16970-1-AP targets NVL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NVL fusion protein Ag9205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear VCP-like
别名 nuclear VCP like, Nuclear VCP like protein, NVL, NVLp
计算分子量856aa,95 kDa; 659aa,73 kDa
观测分子量 95 kDa
GenBank蛋白编号BC012105
基因名称 NVL
Gene ID (NCBI) 4931
RRIDAB_2157811
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15381
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NVL antibody 16970-1-APDownload protocol
IF protocol for NVL antibody 16970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
View Article
humanIF

J Cell Biol

Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.

Authors - Yun Chawon C
View Article
humanWB,IF

Nat Commun

Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins

Authors - Arthur Louche
View Article