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NVL Polyclonal antibody

NVL Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16970-1-AP

Print datasheet

Synonyms

nuclear VCP like, Nuclear VCP like protein, NVL, NVLp

验证数据展示

  • WB analysis using 16970-1-AP

    WB analysis using 16970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16970-1-AP

    IF Staining of HepG2 using 16970-1-AP

    Immunofluorescent analysis of HepG2 cells using 16970-1-AP (NVL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inmouse thymus tissue, mouse lung tissue, HeLa cells, mouse kidney tissue, rat thymus tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16970-1-AP targets NVL in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NVL fusion protein Ag9205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear VCP-like
Synonyms nuclear VCP like, Nuclear VCP like protein, NVL, NVLp
Calculated Molecular Weight856aa,95 kDa; 659aa,73 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC012105
Gene Symbol NVL
Gene ID (NCBI) 4931
RRIDAB_2157811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for NVL antibody 16970-1-APDownload protocol
IF protocol for NVL antibody 16970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
View Article
humanIF

J Cell Biol

Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.

Authors - Yun Chawon C
View Article
humanWB,IF

Nat Commun

Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins

Authors - Arthur Louche
View Article