- Featured Product
- KD/KO Validated
NUPR1 Polyclonal antibody
NUPR1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human colon cancer tissue, human prostate cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NUPR1 Ready-To-Use IHC Kit. NUPR1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15056-1-AP targets NUPR1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | NUPR1 fusion protein Ag7039 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nuclear protein 1 |
| Synonyms | Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8 |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 8 kDa, 17 kDa, 38 kDa |
| GenBank Accession Number | BC002434 |
| Gene Symbol | NUPR1 |
| Gene ID (NCBI) | 26471 |
| RRID | AB_2878104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60356 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUPR1 antibody 15056-1-AP | Download protocol |
| IHC protocol for NUPR1 antibody 15056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma
| ||
J Inorg Biochem Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression
| ||
PLoS One Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells. | ||
Cell Death Discov The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis
| ||
PPAR Res Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
