×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 12329-1-AP (same clone as 12329-1-PBS)

    WB analysis using 12329-1-AP (same clone as 12329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 12329-1-AP (same clone as 12329-1-PBS)

    IP experiment of COLO 320 using 12329-1-AP (same clone as 12329-1-PBS)

    IP result of anti-NUP98-NUP96 (IP:12329-1-AP, 4ug; Detection:12329-1-AP 1:1000) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12329-1-AP (same clone as 12329-1-PBS)

    IF Staining of MCF-7 using 12329-1-AP (same clone as 12329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP98-NUP96 antibody (12329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

NUP98-NUP96 Polyclonal antibody, PBS Only

NUP98-NUP96 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12329-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

12329-1-PBS targets NUP98-NUP96 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3016

Product name: Recombinant human NUP98-NUP96 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1515-1817 aa of BC012906

Sequence: TADPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHMESDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIRVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRVVLSLHHPPDRTSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoporin 98kDa
别名
计算分子量 98 kDa
观测分子量 105 kDa
GenBank蛋白编号BC012906
基因名称 NUP98
Gene ID (NCBI) 4928
RRIDAB_10973678
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52948
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nup98-Nup96 (also known as Nup196, ADIR2 or ADAR2), encoded by NUP98 gene, is a component of the nuclear pore complex (NPC) . It is first synthesized as 186-198 kDa Nup98-Nup96 precursors and the precursors shortly undergo autoproteolysis to give rise to two nucleoporins, the amino-terminal Nup98 (Nup98-N) and carboxy-terminal Nup96 (Nup96-C). Both of Nup98 and Nup96 are localized to the nucleoplasmic side of the NPC. This antibody was raised against the carboxy-terminal region of Nup98-Nup96, it recognizes all Nup98-Nup96 proteins except Nup98-N. Since the autoproteolysis is very rapid this antibody usually detected 98-115 kDa Nup96 protein in multiple cell lines. It is notable that RNA-editing deaminase 1 (Gene ID: 104), also named ADAR2, is a different protein from Nup98-Nup96 (ADAR2).

||
New chat

Able™

正在加载，请稍候...