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验证数据展示

  • Cytometric bead array standard curve of MP00330-1

    Cytometric bead array standard curve of MP00330-1

    Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

  • Sandwich ELISA standard curve of MP00330-1

    Sandwich ELISA standard curve of MP00330-1

    Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00330-1

    Sample test of MP00330-1

    The mean NUP85 concentration was determined to be 1,874.5 pg/mL in mouse liver tissue extract based on a 3.0 mg/mL extract load.

  • WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

    WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

  • WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

    WB analysis using 83288-2-RR (same clone as 83288-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83288-2-RR (NUP85 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83288-2-RR (same clone as 83288-2-PBS)

    FC experiment of MCF-7 using 83288-2-RR (same clone as 83288-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-2-RR, Clone:240116E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83288-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83288-2-RR

    Affinity and Kinetic Characterization of 83288-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83288-2-RR against Human NUP85 were performed. The affinity constant is 27.3 nM.

NUP85 Recombinant antibody, PBS Only (Capture)

NUP85 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83288-2-PBS

产品说明书

CloneNo. 240116E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

240116E10, 85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NUP85 Ready-To-Use IHC Kit. NUP85 primary antibody included.

产品信息

83288-2-PBS targets NUP85 as part of a matched antibody pair:

MP00330-1: 83288-2-PBS capture and 83288-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 NUP85 fusion protein Ag7086 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleoporin 85kDa
别名 240116E10, 85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75
计算分子量 75 kDa
观测分子量70 kDa
GenBank蛋白编号BC000697
基因名称 NUP85
Gene ID (NCBI) 79902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW27
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.