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验证数据展示

  • WB analysis of HeLa using 16232-1-AP (same clone as 16232-1-PBS)

    WB analysis of HeLa using 16232-1-AP (same clone as 16232-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

    IF Staining of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 16232-1-AP (same clone as 16232-1-PBS)

    IHC staining of human stomach using 16232-1-AP (same clone as 16232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

NUP54 Polyclonal antibody, PBS Only

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16232-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16232-1-PBS targets NUP54 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8882

Product name: Recombinant human NUP54 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC012559

Sequence: MAFNFGAPSGTSGTAAATAAPAGGFGGFGTTSTTAGSAFSFSAPTNTGTTGLFGGTQNKGFGFGTGFGTTTGTSTGLGTGLGTGLGFGGFNTQQQQQTTLGGLFSQPTQAPTQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGYFNNNIPPVEFTQENPFCRFKAVGYSCMPSNKDEDGLVVLVFNKKETEIRSQQQQLVESLHKVLGGNQTLTVNVEGTKTLPDDQTEVVIYVVERSPNGTSRRVPATTLYAHFEQANIKTQLQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFKELLRRLKVQDQMTKQHQTRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoporin 54kDa
别名 Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin
计算分子量 507 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC012559
基因名称 NUP54
Gene ID (NCBI) 53371
RRIDAB_10644484
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3B4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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