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验证数据展示

  • WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUP50 antibody included
  • WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

    WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NUP50 Monoclonal antibody, PBS Only

NUP50 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67001-1-PBS

产品说明书

CloneNo. 1D5A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.

产品信息

67001-1-PBS targets NUP50 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15144

Product name: Recombinant human NUP50 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 141-440 aa of BC028125

Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nucleoporin 50kDa
别名 1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50
计算分子量 468 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC028125
基因名称 NUP50
Gene ID (NCBI) 10762
RRIDAB_2882318
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKX7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.