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验证数据展示

  • WB analysis of HeLa using 16084-1-AP

    WB analysis of HeLa using 16084-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16084-1-AP (NUP160 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16084-1-AP

    IP experiment of HeLa using 16084-1-AP

    IP result of anti-NUP160 (IP:16084-1-AP, 4ug; Detection:16084-1-AP 1:500) with HeLa cells lysate 4000ug.

NUP160 Polyclonal antibody

NUP160 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

160 kDa nucleoporin, KIAA0197, nucleoporin 160kDa, Nucleoporin Nup160, NUP120, NUP160

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16084-1-AP targets NUP160 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUP160 fusion protein Ag9083 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoporin 160kDa
别名 160 kDa nucleoporin, KIAA0197, nucleoporin 160kDa, Nucleoporin Nup160, NUP120, NUP160
计算分子量 1316 aa, 149 kDa
观测分子量 149 kDa
GenBank蛋白编号BC009822
基因名称 NUP160
Gene ID (NCBI) 23279
RRIDAB_2157341
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12769
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUP160 antibody 16084-1-APDownload protocol
IP protocol for NUP160 antibody 16084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols