验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16084-1-AP targets NUP160 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9083 Product name: Recombinant human NUP160 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC009822 Sequence: MAAAGALERSFVELSGAERERPRHFREFTVCSIGTANAVAGAVKYSESAGGFYYVESGKLFSVTRNRFIHWKTSGDTLELMEESLDINLLNNAIRLKFQNCSVLPGGVYVSETQNRVIILMLTNQTVHRLLLPHPSRMYRSVSWLSAISFISQITLGVTNVVLERCLLELKEIWILVIPHQAYFDSYRLK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleoporin 160kDa |
| 别名 | 160 kDa nucleoporin, KIAA0197, nucleoporin 160kDa, Nucleoporin Nup160, NUP120, NUP160 |
| 计算分子量 | 1316 aa, 149 kDa |
| 观测分子量 | 149 kDa |
| GenBank蛋白编号 | BC009822 |
| 基因名称 | NUP160 |
| Gene ID (NCBI) | 23279 |
| RRID | AB_2157341 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for NUP160 antibody 16084-1-AP | Download protocol |
| WB protocol for NUP160 antibody 16084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
