验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy NUP133 Ready-To-Use IHC Kit. NUP133 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85609-1-RR targets NUP133 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NUP133 fusion protein Ag3100 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nucleoporin 133kDa |
| 别名 | 133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133 |
| 计算分子量 | 1156 aa, 129 kDa |
| 观测分子量 | 129 kDa |
| GenBank蛋白编号 | BC020107 |
| 基因名称 | NUP133 |
| Gene ID (NCBI) | 55746 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WUM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP133 antibody 85609-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |