验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NUP133 Ready-To-Use IHC Kit. NUP133 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| ChIP | See 1 publications below |
产品信息
12405-1-AP targets NUP133 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | NUP133 fusion protein Ag3100 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleoporin 133kDa |
| 别名 | 133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133, NUP133 |
| 计算分子量 | 1156 aa, 129 kDa |
| 观测分子量 | 129 kDa |
| GenBank蛋白编号 | BC020107 |
| 基因名称 | NUP133 |
| Gene ID (NCBI) | 55746 |
| RRID | AB_2154450 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP133 antibody 12405-1-AP | Download protocol |
| IHC protocol for NUP133 antibody 12405-1-AP | Download protocol |
| IP protocol for NUP133 antibody 12405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Fetal Zone Steroids and Estrogen Show Sex Specific Effects on Oligodendrocyte Precursor Cells in Response to Oxidative Damage. | ||
Mol Cell Pediatr Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs. |
