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NUMB Monoclonal antibody
NUMB Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2G12B7
验证数据展示
经过测试的应用
Positive WB detected in | Human brain tissue, |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60137-1-Ig targets NUMB in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | numb homolog (Drosophila) |
Synonyms | h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171 |
Calculated Molecular Weight | 71 kDa |
Observed Molecular Weight | 66-72 kDa |
GenBank Accession Number | BC068476 |
Gene Symbol | NUMB |
Gene ID (NCBI) | 8650 |
RRID | AB_10598775 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P49757 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NUMB antibody 60137-1-Ig | Download protocol |
IHC protocol for NUMB antibody 60137-1-Ig | Download protocol |
IF protocol for NUMB antibody 60137-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Sci Rep Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis.
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Neurosci Lett Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors.
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Mol Biol Rep Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5. | ||
Stem Cell Res Ther BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction | ||
Cell Rep Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas |