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验证数据展示

  • WB analysis of Jurkat using 16098-1-AP

    WB analysis of Jurkat using 16098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 16098-1-AP

    IHC staining of human heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 16098-1-AP

    IHC staining of human heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of Jurkat using 16098-1-AP

    WB analysis of Jurkat using 16098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16098-1-AP

    IHC staining of human colon cancer using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16098-1-AP

    IHC staining of human colon cancer using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16098-1-AP

    IHC staining of mouse heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16098-1-AP

    IHC staining of mouse heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NUDT8 Polyclonal antibody

NUDT8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16098-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:3.6.1.-, Mitochondrial coenzyme A diphosphatase NUDT8, Nucleoside diphosphate-linked moiety X motif 8, Nudix motif 8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue, human kidney tissue, human skin tissue, mouse heart tissue, human liver tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16098-1-AP targets NUDT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8945

Product name: Recombinant human NUDT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-141 aa of BC018644

Sequence: MLPDCLSAEGELRCRRLLAGATARLRARPASAAVLVPLCSVRGVPALLYTLRSSRLTGRHKGDVSFPGGKCDPADQDVVHTALRETREELGLAVPEEHVWGLLRPVYDPQKATVVPVLAGVGPLDPQSLRPNSEEVSWGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 8
别名 EC:3.6.1.-, Mitochondrial coenzyme A diphosphatase NUDT8, Nucleoside diphosphate-linked moiety X motif 8, Nudix motif 8
计算分子量 236 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC018644
基因名称 NUDT8
Gene ID (NCBI) 254552
RRIDAB_2154132
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WV74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT8 belongs to the Nudix hydrolase family, which is involved in intracellular metabolic processes, especially related to the decomposition and degradation of nucleotides. NUDT8 is a novel CoA diphosphohydrolase that localizes to the mitochondria and is broadly expressed in mitochondria-rich organs such as the heart, kidneys, liver, and brown adipose tissue(PMID: 31004344).

实验方案

Product Specific Protocols
IHC protocol for NUDT8 antibody 16098-1-APDownload protocol
WB protocol for NUDT8 antibody 16098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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