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验证数据展示

  • WB analysis using 34385-1-AP

    WB analysis using 34385-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34385-1-AP (NUDT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 34385-1-AP

    IP experiment of mouse liver using 34385-1-AP

    IP result of anti-NUDT7 (IP:34385-1-AP, 4ug; Detection:34385-1-AP 1:4000) with mouse liver tissue lysate 2480 ug.

NUDT7 Polyclonal antibody

NUDT7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 34385-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Nucleoside diphosphate-linked moiety X motif 7, Nucleoside diphosphate-linked moiety X motif 7 (Nudix motif 7), Peroxisomal coenzyme A diphosphatase NUDT7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse liver tissue
Positive IP detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

34385-1-AP targets NUDT7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag41199

Product name: Recombinant human NUDT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 37-172 aa of NM_001105663

Sequence: YNKYSVLLPLVAKEGKLHLLFTVRSEKLRRAPGEVCFPGGKRDPTDMDDAATALREAQEEVGLRPHQVEVVCCLVPCLIDTDTLITPFVGLIDHNFQAQPNPAEVKDVFLVPLAYFLHPQVHDQHYVTRLGHRFIN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 7
别名 Nucleoside diphosphate-linked moiety X motif 7, Nucleoside diphosphate-linked moiety X motif 7 (Nudix motif 7), Peroxisomal coenzyme A diphosphatase NUDT7
计算分子量238aa, 27 kDa
观测分子量30 kDa
GenBank蛋白编号NM_001105663
基因名称 NUDT7
Gene ID (NCBI) 283927
RRIDAB_3743196
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP0C024
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxisomal coenzyme A diphosphatase NUDT7, a peroxisomal Nudix hydrolase, mainly localizes to the peroxisomal matrix and catalyzes hydrolysis of CoA/acyl-CoAs, regulating lipid metabolism and CoA homeostasis (PMID: 11415433; 25110012). Its dysregulation links to diabetes, Kras-driven colorectal cancer, neurodegeneration with brain iron accumulation, and osteoarthritis (PMID: 32131398; 24847269; 30143643).

实验方案

Product Specific Protocols
IP protocol for NUDT7 antibody 34385-1-APDownload protocol
WB protocol for NUDT7 antibody 34385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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