验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue, mouse brain tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1638 Product name: Recombinant human NUDT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC009842 Sequence: MRQPLSWGRWRAMLARTYGPGPSAGYRWASGAQGYVRNPPVGACDLQGELDRFGGISVRLARLDALDRLDAAAFQKGLQAAVQQWRSEGRTAVWLHIPILQSRFIAPAASLGFCFHHAESDSSTLTLWLREGPSRLPGYASHQVGVAGAVFDESTRKILVVQDRNKLKNMWKFPGGLSEPEEDIGDTAVREVFEETGIKSEFRSVLSIRQQHTNPGAFGKSDMYIICRLKPYSFTINFCQEECLRCEWMDLNDLAKTENTTPITSRVARLLLYGYREGFDKIDLTVEELPAVYTGLFYKLYHKELPENYKTMKGID 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 6 |
| 别名 | gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.- |
| 计算分子量 | 36 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC009842 |
| 基因名称 | NUDT6 |
| Gene ID (NCBI) | 11162 |
| RRID | AB_2236190 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53370 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NUDT6 antibody 11181-1-AP | Download protocol |
| IHC protocol for NUDT6 antibody 11181-1-AP | Download protocol |
| IP protocol for NUDT6 antibody 11181-1-AP | Download protocol |
| WB protocol for NUDT6 antibody 11181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Physiol FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling | ||
Mol Endocrinol Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. | ||
Mol Carcinog Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. | ||
Genes (Basel) Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.
| ||
Wound Repair Regen GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. |
