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验证数据展示

  • WB analysis using 27004-1-AP (same clone as 27004-1-PBS)

    WB analysis using 27004-1-AP (same clone as 27004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27004-1-AP (NUDT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 27004-1-AP (same clone as 27004-1-PBS)

    IHC staining of mouse liver using 27004-1-AP (same clone as 27004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27004-1-AP (NUDT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 27004-1-AP (same clone as 27004-1-PBS)

    IF Staining of HeLa using 27004-1-AP (same clone as 27004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT5 antibody (27004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27004-1-PBS in a different storage buffer formulation.

NUDT5 Polyclonal antibody, PBS Only

NUDT5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27004-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27004-1-PBS targets NUDT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25485

Product name: Recombinant human NUDT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC000025

Sequence: MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 5
别名
计算分子量 24 kDa
观测分子量 35 kDa
GenBank蛋白编号BC000025
基因名称 NUDT5
Gene ID (NCBI) 11164
RRIDAB_3085919
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKK9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUDT5 (NUDIX hydrolase type 5), also called NUDIX5, is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 expression can affect chromosome remodeling, involved in cell adhesion, cancer stem cell maintenance and epithelial to mesenchyme transition in breast cancer cells. In addtion, the high expression of NUDT5 is probably involved in the poor prognosis of breast cancer, which could be a prognostic factor and potential target in the diagnosis and treatment of breast cancer (PMID: 33571243). This NUDT5 protein can be detected with a molecular weight of 35 kDa (PMID: 33096144).

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