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  • Featured Product
  • KD/KO Validated

NUDT16 Polyclonal antibody

NUDT16 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12889-1-AP

Print datasheet

Synonyms

Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme

验证数据展示

  • WB analysis using 12889-1-AP

    WB analysis using 12889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12889-1-AP (NUDT16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12889-1-AP

    IP experiment of HEK-293 using 12889-1-AP

    IP result of anti-NUDT16 (IP:12889-1-AP, 3ug; Detection:12889-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

  • IHC staining of human kidney using 12889-1-AP

    IHC staining of human kidney using 12889-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12889-1-AP (NUDT16 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 12889-1-AP

    IHC staining of human kidney using 12889-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12889-1-AP (NUDT16 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 12889-1-AP

    IF Staining of HEK-293 using 12889-1-AP

    Immunofluorescent analysis of HEK-293 cells using 12889-1-AP (NUDT16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12889-1-AP targets NUDT16 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen NUDT16 fusion protein Ag3925 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 16
Synonyms Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme
Calculated Molecular Weight 195 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC031215
Gene Symbol NUDT16
Gene ID (NCBI) 131870
RRIDAB_10640536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUDT16 antibody 12889-1-APDownload protocol
IHC protocol for NUDT16 antibody 12889-1-APDownload protocol
IF protocol for NUDT16 antibody 12889-1-APDownload protocol
IP protocol for NUDT16 antibody 12889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.

Authors - Siddharth Shukla
  • KD Validated
View Article
WB

Mol Biol Cell

L-bodies are RNA-protein condensates driving RNA localization in Xenopus oocytes.

Authors - Christopher R Neil
View Article
humanIHC

Front Immunol

m7G regulator-mediated methylation modification patterns define immune cell infiltration and patient survival

Authors - Lu Wang
View Article
humanWB

Nucleic Acids Res

NUDT16 regulates CtIP PARylation to dictate homologous recombination repair

Authors - Zhen Zhang
  • KO Validated
View Article