验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human rectal cancer tissue, mouse lung tissue, mouse stomach tissue, mouse skeletal muscle tissue, human stomach cancer tissue, mouse adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy NUDT16 Ready-To-Use IHC Kit. NUDT16 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 5 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
12889-1-AP targets NUDT16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3925 Product name: Recombinant human NUDT16 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-195 aa of BC031215 Sequence: MAGARRLELGEALALGSGWRHVCHALLYAPDPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREELGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRAKDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGLLQSGSISGLKIPAHH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 16 |
| 别名 | EC:3.6.1.62, EC:3.6.1.64, IDP phosphatase, IDPase, Inosine diphosphate phosphatase |
| 计算分子量 | 195 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC031215 |
| 基因名称 | NUDT16 |
| Gene ID (NCBI) | 131870 |
| RRID | AB_10640536 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96DE0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| IHC protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| WB protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| IP protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Nudix hydrolase NUDT16 regulates 53BP1 protein by reversing 53BP1 ADP-ribosylation. | ||
Redox Biol Overexpression of NUDT16L1 sustains proper function of mitochondria and leads to ferroptosis insensitivity in colorectal cancer
| ||
Mol Cell 5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.
| ||
Nucleic Acids Res NUDT16 regulates CtIP PARylation to dictate homologous recombination repair
| ||
Proc Natl Acad Sci U S A Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.
| ||
Front Immunol Analysis of m7G-related signatures in the tumor immune microenvironment and identification of clinical prognostic regulators in ovarian cancer.
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