验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25914-1-AP targets NUDT15 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23242 Product name: Recombinant human NUDT15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC133015 Sequence: MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAALHLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRCLKEQGYDPFKEDLNHLVGYKGNHL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 15 |
| 别名 | 8 oxo dGTPase NUDT15, EC:3.6.1.9, MTH2, MutT homolog 2, Nucleoside diphosphate-linked moiety X motif 15 |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC133015 |
| 基因名称 | NUDT15 |
| Gene ID (NCBI) | 55270 |
| RRID | AB_2880294 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NV35 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUDT15 (nucleoside diphosphate-linked moiety X-type motif 15) is homologues to NUDT1 (MutT homologue 1, MTH1) and thus has previously been referred to as MTH2. NUDT15 is a key enzyme in thiopurine metabolism and tempers the activity of thiopurine drugs by catalyzing the hydrolysis of 6-thio-dGTP and 6-thio-GTP (PMID: 27530327, 32690945). NUDT15 is a 164-amino-acid protein that belongs to the nudix hydrolase enzyme family, whose members are capable of hydrolyzing compounds with the general structure of a nucleoside diphosphate linked to another moiety, X. (PMID: 25108385)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUDT15 antibody 25914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
