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验证数据展示

  • WB analysis using 17487-1-AP

    WB analysis using 17487-1-AP

    mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17487-1-AP

    IF Staining of HeLa using 17487-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 17487-1-AP

    IF Staining of MCF-7 using 17487-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17487-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

DeNADding enzyme NUDT12, EC:3.6.1.-, EC:3.6.1.22, NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NUDT12 fusion protein Ag11557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 12
别名 DeNADding enzyme NUDT12, EC:3.6.1.-, EC:3.6.1.22, NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12
计算分子量 462 aa, 52 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC041099
基因名称 NUDT12
Gene ID (NCBI) 83594
RRIDAB_2236164
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT12 is a member of the Nudix superfamily of pyrophosphohydrolases that has a broad range of substrates, including (d)NTPs, nucleotides, capped RNAs, dinucleotide coenzymes (NAD, FAD, dpCoA), and their derivatives. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets (PMID: 37301333, 31875550). The NUDT12 gene on chromosome 5q21 encodes the open reading frame of the putative 462-amino-acid, 52 kDa NUDT12 protein (PMID: 12790796).

实验方案

Product Specific Protocols
WB protocol for NUDT12 antibody 17487-1-APDownload protocol
IF protocol for NUDT12 antibody 17487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.

Authors - Hiroaki Konishi
View Article