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NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17487-1-AP

Print datasheet

Synonyms

Nudix motif 12, NUDT12

验证数据展示

  • WB analysis using 17487-1-AP

    WB analysis using 17487-1-AP

    mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17487-1-AP

    IF Staining of HeLa using 17487-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17487-1-AP (NUDT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17487-1-AP targets NUDT12 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NUDT12 fusion protein Ag11557 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 12
Synonyms Nudix motif 12, NUDT12
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC041099
Gene Symbol NUDT12
Gene ID (NCBI) 83594
RRIDAB_2236164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUDT12 antibody 17487-1-APDownload protocol
IF protocol for NUDT12 antibody 17487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.

Authors - Hiroaki Konishi
View Article