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NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17508-1-AP

Print datasheet

Synonyms

APS2, DIPP 3 alpha, DIPP3 alpha, DIPP3a, hAps2, hDIPP3alpha, Nudix motif 10, NUDT10

验证数据展示

  • WB analysis using 17508-1-AP

    WB analysis using 17508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17508-1-AP

    IF Staining of HeLa using 17508-1-AP

    Immunofluorescent analysis of Hela cells, using NUDT10 antibody 17508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17508-1-AP targets NUDT3/4/10/11 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen NUDT3/4/10/11 fusion protein Ag11711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 10
Synonyms APS2, DIPP 3 alpha, DIPP3 alpha, DIPP3a, hAps2, hDIPP3alpha, Nudix motif 10, NUDT10
Calculated Molecular Weight 164 aa, 19 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC050700
Gene Symbol NUDT10
Gene ID (NCBI) 170685
RRIDAB_2154145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

实验方案

Product Specific Protocols
WB protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
IF protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols