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验证数据展示

  • WB analysis using 32473-1-AP

    WB analysis using 32473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32473-1-AP (NUDCD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 32473-1-AP

    IF Staining of A431 using 32473-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NUDCD1 antibody (32473-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

NUDCD1 Polyclonal antibody

NUDCD1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32473-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Tumor antigen CML66, NudC domain-containing protein 1, CML66, Chronic myelogenous leukemia tumor antigen 66

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32473-1-AP targets NUDCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38518

Product name: Recombinant human NUDCD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-498 aa of NM_032869.3

Sequence: QQTEDDLTVTIRLPEDSTKEDIQIQFLPDHINIVLKDHQFLEGKLYSSIDHESSTWIIKESNSLEISLIKKNEGLTWPELVIGDKQGELIRDSAQCAAIAERLMHLTSEELNPNPDKEKPPCNAQELEECDIFFEESSSLCRFDGNTLKTTHVVNLGSNQYLFSVIVDPKEMPCFCLRHDVDALLWQPHSSKQDDMWEHIATFNALGYVQASKRDKKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NudC domain containing 1
别名 Tumor antigen CML66, NudC domain-containing protein 1, CML66, Chronic myelogenous leukemia tumor antigen 66
计算分子量67 kDa，583aa
观测分子量67 kDa
GenBank蛋白编号NM_032869.3
基因名称 NUDCD1
Gene ID (NCBI) 84955
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96RS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NudC domain-containing protein 1 (NUDCD1, also known as CML66) is a protein that plays a crucial role in various biological processes, including cell proliferation, migration, cell cycle progression, and intracellular material transport (PMID: 38553373). NUDCD1 has been identified as an oncoprotein frequently activated or overexpressed in multiple human cancers, suggesting its role in promoting cancer cell growth and metastasis (PMID: 29888104). It is abnormally activated in various tumors and has been identified as a cancer antigen (PMID: 37338527).

实验方案

Product Specific Protocols
IF protocol for NUDCD1 antibody 32473-1-APDownload protocol
WB protocol for NUDCD1 antibody 32473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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