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验证数据展示

  • WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

    WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

    IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUCKS1 antibody included
  • WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

    WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

  • IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

    IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUCKS1 antibody included
  • IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

    IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUCKS1 antibody included
  • IP experiment of HEK-293 using 12023-2-AP (same clone as 12023-2-PBS)

    IP experiment of HEK-293 using 12023-2-AP (same clone as 12023-2-PBS)

    IP result of anti-NUCKS1 (IP:12023-2-AP, 4ug; Detection:12023-2-AP 1:600) with HEK-293 cells lysate 960 ug. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NUCKS1 Polyclonal antibody, PBS Only

NUCKS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12023-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NUCKS1 Ready-To-Use IHC Kit. NUCKS1 primary antibody included.

产品信息

12023-2-PBS targets NUCKS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag2652

Product name: Recombinant human NUCKS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-243 aa of BC000805

Sequence: MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKKDDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDEEPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear casein kinase and cyclin-dependent kinase substrate 1
别名
计算分子量 243 aa, 27 kDa
观测分子量 45 kDa
GenBank蛋白编号BC000805
基因名称 NUCKS1
Gene ID (NCBI) 64710
RRIDAB_906407
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1E3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.

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