验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse pancreas tissue, L02 cells, U-251 cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human skeletal muscle tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
15409-1-AP targets NUBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7676 Product name: Recombinant human NUBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC002768 Sequence: MEAAAEPGNLAGVRHIILVLSGKGGVGKSTISTELALALRHAGKKVGILDVDLCGPSIPRMLGAQGRAVHQCDRGWAPVFLDREQSISLMSVGFLLEKPDEAVVWRGPKKNALIKQFVSDVAWGELDYLVVDTPPGTSDEHMATIEALRPYQPLGALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCTECTSVFSRGGGEELAQLAGVPFLGSVPLDPALMRTLEEGHDFIQEFPGSPAFAALTSIAQKILDATPACLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleotide binding protein 2 (MinD homolog, E. coli) |
| 别名 | Nucleotide-binding protein 2, Nucleotide binding protein 2, NUBP1, NBP 2, Cytosolic Fe-S cluster assembly factor NUBP2 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 30-33 kDa |
| GenBank蛋白编号 | BC002768 |
| 基因名称 | NUBP2 |
| Gene ID (NCBI) | 10101 |
| RRID | AB_10858630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NUBP2 antibody 15409-1-AP | Download protocol |
| IHC protocol for NUBP2 antibody 15409-1-AP | Download protocol |
| WB protocol for NUBP2 antibody 15409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators. | ||
J Biol Chem Iron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery. | ||
Mol Cell Biochem Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer |
