验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse brain tissue, rat heart tissue, rat kidney tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20235-1-AP targets NTN1-Specific in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | netrin 1 |
| 别名 | NTN1, Epididymis tissue protein Li 131P, netrin 1, Netrin-1, NTN1L |
| 计算分子量 | 68 kDa |
| 观测分子量 | 65-68 kDa |
| GenBank蛋白编号 | NM_004822 |
| 基因名称 | NTN1 |
| Gene ID (NCBI) | 9423 |
| RRID | AB_2918065 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95631 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NTN1 is also named as NTN1L. Netrins control the guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor through its association with its receptors, which prevents the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NTN1-Specific antibody 20235-1-AP | Download protocol |
| WB protocol for NTN1-Specific antibody 20235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Eupatilin inhibits non-small cell lung cancer metastasis by suppressing Netrin-1-mediated epithelial-mesenchymal transition. | ||
Transl Psychiatry mPFC DCC coupling with CaMKII+ neuronal excitation participates in behavioral despair in male mice | ||
Int J Oncol Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer. | ||
J Ethnopharmacol Jiawei Qingxin Zishen Decoction mitigates granulosa cell apoptosis and enhances ovarian reserve through Netrin/UNC5B signaling pathway. | ||
J Ethnopharmacol Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses | ||
Int Immunopharmacol The regulatory role of the Netrin-1/UNC5H3 pathway in neuronal pyroptosis after stroke |
