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验证数据展示

  • WB analysis of SH-SY5Y using 14373-1-AP

    WB analysis of SH-SY5Y using 14373-1-AP

    WB result of NTM antibody (14373-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NTM transfected SH-SY5Y cells.

  • WB analysis of SH-SY5Y using 14373-1-AP

    WB analysis of SH-SY5Y using 14373-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14373-1-AP

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14373-1-AP

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NTM Polyclonal antibody

NTM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14373-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14373-1-AP targets NTM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5746

Product name: Recombinant human NTM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-316 aa of BC050716

Sequence: VCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDNRVTRVAWLNRSTILYAGNDKWCLDPRVVLLSNTQTQYSIEIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVSPKIVEISSDISINEGNNISLTCIATGRPEPTVTWRHISPKAVGFVSEDEYLEIQGITREQSGDYECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSAEFQWYKDDKRLIEGKKGVKVENRPFLSKLIFFNVSEHDYGNYTCVASNKLGHTNASIMLFGETVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurotrimin
别名 HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI
计算分子量 38 kDa
观测分子量 58 kDa, 65 kDa
GenBank蛋白编号BC050716
基因名称 NTM
Gene ID (NCBI) 50863
RRIDAB_2878048
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.

实验方案

Product Specific Protocols
IHC protocol for NTM antibody 14373-1-APDownload protocol
WB protocol for NTM antibody 14373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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