验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue |
| Positive IF/ICC detected in | PC-3 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12297-1-AP targets Neurotrophin 4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2938 Product name: Recombinant human NTF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC012421 Sequence: MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurotrophin 4 |
| 别名 | NTF4, NT-4, NT4, NT 4, Neutrophic factor 4 |
| 计算分子量 | 210 aa, 22 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC012421 |
| 基因名称 | Neurotrophin 4 |
| Gene ID (NCBI) | 4909 |
| RRID | AB_10598309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34130 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
| WB protocol for Neurotrophin 4 antibody 12297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Oncol Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.
| ||
