验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, L02 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17132-1-AP targets PNAD in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PNAD fusion protein Ag10880 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-terminal asparagine amidase |
| 别名 | NTAN1, EC:3.5.1.121, N terminal asparagine amidase, PNAA, Protein NH2-terminal asparagine amidohydrolase |
| 计算分子量 | 310 aa, 35 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC017336 |
| 基因名称 | NTAN1 |
| Gene ID (NCBI) | 123803 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AB6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNAD is an N-terminal aspartic acid deamidation enzyme, which can deamidate N-terminal aspartic acid residues to aspartic acid, making the protein prone to arginination, polyubiquitination and degradation. In addition, PNAD is involved in the regulation of apoptosis by mediating the degradation of the C-terminal fragment of Drosophila apoptosis suppressor protein 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNAD antibody 17132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |