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验证数据展示

  • WB analysis using 20765-1-AP

    WB analysis using 20765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 20765-1-AP

    IP experiment of mouse skeletal muscle using 20765-1-AP

    IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

NT5M Polyclonal antibody

NT5M Polyclonal Antibody for WB, IP, ELISA
Cat No. 20765-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IP, ELISA

别名

5'(3')-deoxyribonucleotidase, mitochondrial, 5',3'-nucleotidase, mitochondrial, Deoxy-5'-nucleotidase 2, dNT 2, DNT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20765-1-AP targets NT5M in WB, IP, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag14743

Product name: Recombinant human NT5M protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-228 aa of BC035838

Sequence: LRVLVDMDGVLADFEGGFLRKFRARFPDQPFIALEDRRGFWVSEQYGRLRPGLSEKAISIWESKNFFFELEPLPGAVEAVKEMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKYFGPDFLEQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQPPRRRLHSWADDWKAILDSKRPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5',3'-nucleotidase, mitochondrial
别名 5'(3')-deoxyribonucleotidase, mitochondrial, 5',3'-nucleotidase, mitochondrial, Deoxy-5'-nucleotidase 2, dNT 2, DNT2
计算分子量 228 aa, 26 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC035838
基因名称 NT5M
Gene ID (NCBI) 56953
RRIDAB_10733338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NT5M is a mitochondrial 5′(3′)-deoxyribonucleotidase that plays a crucial role in maintaining balanced deoxynucleotide pools essential for mitochondrial DNA synthesis. It dephosphorylates specific nucleoside monophosphates, particularly dUMP and dTMP, thereby preventing the accumulation of toxic dTTP. NT5M is predominantly localized in mitochondria and is highly expressed in tissues such as the heart, brain, and skeletal muscle.

实验方案

Product Specific Protocols
WB protocol for NT5M antibody 20765-1-APDownload protocol
IP protocol for NT5M antibody 20765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols