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验证数据展示

  • WB analysis of HepG2 using 67789-1-Ig (same clone as 67789-1-PBS)

    WB analysis of HepG2 using 67789-1-Ig (same clone as 67789-1-PBS)

    WB result of NT5E/CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • WB analysis using 67789-1-Ig (same clone as 67789-1-PBS)

    WB analysis using 67789-1-Ig (same clone as 67789-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CD73 Monoclonal antibody, PBS Only

CD73 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67789-1-PBS

产品说明书

CloneNo. 1G5G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

NT5E, 1G5G8, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67789-1-PBS targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30616

Product name: Recombinant human NT5E,CD73 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 32-264 aa of BC015940

Sequence: LHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNCFKRIAWARMSR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 1G5G8, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)
计算分子量 29 kDa, 63 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC015940
基因名称 CD73
Gene ID (NCBI) 4907
RRIDAB_2918553
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP21589
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

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