验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, SW480 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31739-1-AP targets NT5DC2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NT5DC2 fusion protein Ag35015 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5'-nucleotidase domain containing 2 |
| 别名 | |
| 计算分子量 | 61 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | NM_022908 |
| 基因名称 | NT5DC2 |
| Gene ID (NCBI) | 64943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H857 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
5'-Nucleotidase domain containing 2 (NT5DC2) is a member of the NT5DC family and contains a haloacid dehalogenase motif localized in the N-terminus of these proteins (PMID: 32962856). NT5DC2 is associated with attention-deficit/hyperactivity disorder and bipolar disorder. NT5DC2 has been shown to interact with and stabilize Fyn, a Src family proto-oncogene, and plays a role in regulating glioblastoma progression. NT5DC2 promotes tumor cell proliferation by stabilizing EGFR in hepatocellular carcinoma (PMID: 32382041).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NT5DC2 antibody 31739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |