验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, mouse kidney tissue, mouse placenta tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11393-1-AP targets NT5C3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag1949 Product name: Recombinant human NT5C3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC015856 Sequence: MTNQESAVHVKMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGALRNTEYFNQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5'-nucleotidase, cytosolic III |
| 别名 | 5' nucleotidase, cytosolic III, cN III, Cytosolic 5 nucleotidase 3, Cytosolic 5 nucleotidase III, NT5C3, p36, P5N 1, P5'N 1, P5N1, PN I, PSN1, Pyrimidine 5 nucleotidase 1, UMPH, UMPH1 |
| 计算分子量 | 297 aa, 34 kDa |
| 观测分子量 | 33-38 kDa |
| GenBank蛋白编号 | BC015856 |
| 基因名称 | NT5C3 |
| Gene ID (NCBI) | 51251 |
| RRID | AB_2267361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0P0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NT5C3 antibody 11393-1-AP | Download protocol |
| IP protocol for NT5C3 antibody 11393-1-AP | Download protocol |
| WB protocol for NT5C3 antibody 11393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
