验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
15223-1-AP targets NT5C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7438 Product name: Recombinant human NT5C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-561 aa of BC001595 Sequence: HYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5'-nucleotidase, cytosolic II |
| 别名 | Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II |
| 计算分子量 | 65 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC001595 |
| 基因名称 | NT5C2 |
| Gene ID (NCBI) | 22978 |
| RRID | AB_2153946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49902 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for NT5C2 antibody 15223-1-AP | Download protocol |
| WB protocol for NT5C2 antibody 15223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Med Genomics Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis. | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation |
