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验证数据展示

  • WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

    WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

  • WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

    WB analysis using 17546-1-AP (same clone as 17546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 17546-1-AP (same clone as 17546-1-PBS)

    IHC staining of rat testis using 17546-1-AP (same clone as 17546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17546-1-AP (NSUN7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17546-1-PBS in a different storage buffer formulation.

NSUN7 Polyclonal antibody, PBS Only

NSUN7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17546-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

FLJ14001

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17546-1-PBS targets NSUN7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11650

Product name: Recombinant human NSUN7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-475 aa of BC036568

Sequence: SLIIVMLYDFQDRKFQTRVLSDNEEPISEVQEVENLLNSFKIKLAAALARCRIKHDALSIYHILPETVRKQELRASTLPLYAWINTCKISPEEVYNNLKRRGYNKVKSVLHIDDKVFAVDQHCYDVLIFPSHLKNDLINIDLFKDYKLIFQDKSRSLAVHSVKALLNMDDDVLMVNTGSWYTVAHMSILTNNNTSKVFVCGVQSQAKDPDLKTLFTKIGCKNIEILHEKFINIESKDHRLQKVKVILLLPRCSGLGVSNPVEFILNEHEDTEFLKDHSQGGISVDKLHVLAQQQYEQLTHAMKFTKAQAVVYCTCSVFPEENEAVVKKALEFQDLGNKGQPYSGTLLRQCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 7
别名 FLJ14001
计算分子量 718 aa, 81 kDa
观测分子量 80 kDa
GenBank蛋白编号BC036568
基因名称 NSUN7
Gene ID (NCBI) 79730
RRIDAB_2878415
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NE18
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

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