• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82894-2-RR (same clone as 82894-2-PBS)

    WB analysis using 82894-2-RR (same clone as 82894-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82894-2-RR (NSUN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82894-2-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    WB analysis of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    WB result of NSUN2 antibody (82894-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HeLa cells. This data was developed using the same antibody clone with 82894-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82894-2-RR (same clone as 82894-2-PBS)

    IF Staining of HepG2 using 82894-2-RR (same clone as 82894-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82894-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    IF Staining of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    FC experiment of HeLa using 82894-2-RR (same clone as 82894-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-2-RR, Clone:230186G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82894-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82894-2-RR

    Affinity and Kinetic Characterization of 82894-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82894-2-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

NSUN2 Recombinant monoclonal antibody, PBS Only

NSUN2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82894-2-PBS

产品说明书

CloneNo. 230186G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

产品信息

82894-2-PBS targets NSUN2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14791

Product name: Recombinant human NSUN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 415-767 aa of BC001041

Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 2
别名 RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-
计算分子量 767 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC001041
基因名称 NSUN2
Gene ID (NCBI) 54888
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08J23
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

||
New chat Able
正在加载，请稍候...