• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NSUN2 Recombinant antibody

NSUN2 Recombinant Antibody for IF/ICC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230186A6

Cat No : 82894-1-RR

Print datasheet

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • FC experiment of HeLa using 82894-1-RR

    FC experiment of HeLa using 82894-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human NSUN2 (82894-1-RR, Clone:230186A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HepG2 using 82894-1-RR

    IF Staining of HepG2 using 82894-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82894-1-RR targets NSUN2 in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC, ELISA
Tested Reactivity Human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

实验方案

Product Specific Protocols
IF protocol for NSUN2 antibody 82894-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols