验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HepG2 cells, LNCaP cells, Raji cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32113-1-AP targets NSMCE1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36841 Product name: Recombinant human NSMCE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC018938 Sequence: RMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRSATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | non-SMC element 1 homolog (S. cerevisiae) |
| 别名 | NSE1, Non-structural maintenance of chromosomes element 1 homolog, Non-SMC element 1 homolog, HSPC337, HSPC333 |
| 计算分子量 | 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC018938 |
| 基因名称 | NSMCE1 |
| Gene ID (NCBI) | 197370 |
| RRID | AB_3670196 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WV22 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Non-structural maintenance of chromosomes element 1 homolog (NSMCE1, also known as NSE1) located in the nucleoplasm, which involves the maintenance of genome integrity, DNA damage response, and DNA repair (PMID: 18086888; 20864041; 29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of the SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PMID: 18086888).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSMCE1 antibody 32113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
