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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 32113-1-AP

    WB analysis using 32113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32113-1-AP (NSMCE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

NSMCE1 Polyclonal antibody

NSMCE1 Polyclonal Antibody for WB, ELISA
Cat No. 32113-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

NSE1, Non-structural maintenance of chromosomes element 1 homolog, Non-SMC element 1 homolog, HSPC337, HSPC333

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HepG2 cells, LNCaP cells, Raji cells, U-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32113-1-AP targets NSMCE1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NSMCE1 fusion protein Ag36841 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 non-SMC element 1 homolog (S. cerevisiae)
别名 NSE1, Non-structural maintenance of chromosomes element 1 homolog, Non-SMC element 1 homolog, HSPC337, HSPC333
计算分子量31 kDa
观测分子量31 kDa
GenBank蛋白编号BC018938
基因名称 NSMCE1
Gene ID (NCBI) 197370
RRIDAB_3670196
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WV22
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Non-structural maintenance of chromosomes element 1 homolog (NSMCE1, also known as NSE1) located in the nucleoplasm, which involves the maintenance of genome integrity, DNA damage response, and DNA repair (PMID: 18086888; 20864041; 29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of the SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PMID: 18086888).

实验方案

Product Specific Protocols
WB protocol for NSMCE1 antibody 32113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols