NSMAF Polyclonal antibody
NSMAF Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19407-1-AP targets NSMAF in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | human |
| Immunogen | NSMAF fusion protein Ag5730 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | neutral sphingomyelinase (N-SMase) activation associated factor |
| Synonyms | FAN, NSMAF, Protein FAN |
| Calculated Molecular Weight | 104 kDa |
| Observed Molecular Weight | 100-104 kDa |
| GenBank Accession Number | BC041124 |
| Gene Symbol | NSMAF |
| Gene ID (NCBI) | 8439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSMAF is a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSMAF antibody 19407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |