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验证数据展示

  • WB analysis using 28837-1-AP

    WB analysis using 28837-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28837-1-AP (NSF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28837-1-AP

    IHC staining of mouse brain using 28837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28837-1-AP (NSF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28837-1-AP

    IHC staining of mouse brain using 28837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28837-1-AP (NSF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NSF Polyclonal antibody

NSF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28837-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28837-1-AP targets NSF in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag30229

Product name: Recombinant human NSF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 95-255 aa of BC030613

Sequence: MTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor
别名 NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF
计算分子量 744 aa, 83 kDa
观测分子量70-83 kDa
GenBank蛋白编号BC030613
基因名称 NSF
Gene ID (NCBI) 4905
RRIDAB_2918208
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The N-ethylmaleimide-sensitive fusion protein (NSF) is a 70-83 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

实验方案

Product Specific Protocols
WB protocol for NSF antibody 28837-1-APDownload protocol
IHC protocol for NSF antibody 28837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols