- Featured Product
- KD/KO Validated
NSDHL Polyclonal antibody
NSDHL Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human heart tissue, mouse brain tissue |
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3200 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 6 publications below |
产品信息
15111-1-AP targets NSDHL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | NSDHL fusion protein Ag7097 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NAD(P) dependent steroid dehydrogenase-like |
| Synonyms | H105E3, NSDHL, Protein H105e3, SDR31E1, XAP104 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa, 40 kDa |
| GenBank Accession Number | BC000245 |
| Gene Symbol | NSDHL |
| Gene ID (NCBI) | 50814 |
| RRID | AB_2155681 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15738 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IHC protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IF protocol for NSDHL antibody 15111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.
| ||
Cancer Discov Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation. | ||
Clin Cancer Res Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.
| ||
Cell Rep Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR. | ||
Cell Cycle Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice. | ||
