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NSBP1 Polyclonal antibody

NSBP1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 23955-1-AP

Print datasheet

Synonyms

HMGN5, NSBP1, nucleosomal binding protein 1, Nucleosome binding protein 1

验证数据展示

  • WB analysis of PC-3 using 23955-1-AP

    WB analysis of PC-3 using 23955-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23955-1-AP

    IHC staining of human testis using 23955-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

  • IF Staining of HeLa using 23955-1-AP

    IF Staining of HeLa using 23955-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23955-1-AP (NSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23955-1-AP is NSBP1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NSBP1 fusion protein Ag20884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleosomal binding protein 1
Synonyms HMGN5, NSBP1, nucleosomal binding protein 1, Nucleosome binding protein 1
Calculated Molecular Weight 282 aa, 32 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005342
Gene Symbol NSBP1
Gene ID (NCBI) 79366
RRIDAB_2879374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP82970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSBP1, also named Nucleosome-binding protein 1 orHMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).

实验方案

Product Specific Protocols
WB protocol for NSBP1 antibody 23955-1-APDownload protocol
IHC protocol for NSBP1 antibody 23955-1-APDownload protocol
IF protocol for NSBP1 antibody 23955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

HMGN5 promotes invasion and migration of colorectal cancer through activating FGF/FGFR pathway.

Authors - G-J Zhu
View Article