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验证数据展示

  • WB analysis using 23805-1-AP

    WB analysis using 23805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23805-1-AP (NRK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 23805-1-AP

    WB analysis using 23805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23805-1-AP (NRK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 23805-1-AP

    IP experiment of mouse kidney using 23805-1-AP

    IP result of anti-NRK1 (IP:23805-1-AP, 4ug; Detection:23805-1-AP 1:3000) with mouse kidney tissue lysate 3400 ug.

NRK1 Polyclonal antibody

NRK1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 23805-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

C9orf95, Nicotinamide riboside kinase 1, NMRK1, Ribosylnicotinic acid kinase 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23805-1-AP targets NRK1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20746

Product name: Recombinant human C9orf95 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-199 aa of BC001366

Sequence: MKTFIIGISGVTNSGKTTLAKNLQKHLPNCSVISQDDFFKPESEIETDKNGFLQYDVLEALNMEKMMSAISCWMESARHSVVSTDQESAEEIPILIIEGFLLFNYKPLDTIWNRSYFLTIPYEECKRRRSTRVYQPPDSPGYFDGHVWPMYLKYRQEMQDITWEVVYLDGTKSEEDLFLQVYEDLIQELAKQKCLQVTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 95
别名 C9orf95, Nicotinamide riboside kinase 1, NMRK1, Ribosylnicotinic acid kinase 1
计算分子量 199 aa, 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC001366
基因名称 C9orf95
Gene ID (NCBI) 54981
RRIDAB_3742091
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NWW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRK1 (Nicotinamide riboside kinase 1) is necessary and rate-limiting for the use of exogenous NR and NMN for NAD þ synthesis. Functionally, NRK1 activity restrains CD4+ T cell activation and cytokine production and promotes survival. These activities are linked to increased NRK1 expression in the cytoplasm, where it locally elevates NAD/H levels (PMID: 27725675, 29678570).

实验方案

Product Specific Protocols
IP protocol for NRK1 antibody 23805-1-APDownload protocol
WB protocol for NRK1 antibody 23805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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