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NRG1, isoform Alpha Polyclonal antibody

NRG1, isoform Alpha Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 31043-1-AP

Print datasheet

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform Alpha, NRG1A, Pro NRG1, SMDF

验证数据展示

  • IF Staining of MCF-7 using 31043-1-AP

    IF Staining of MCF-7 using 31043-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 31043-1-AP

    WB analysis using 31043-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31043-1-AP targets NRG1, isoform Alpha in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen NRG1, isoform Alpha fusion protein Ag35114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neuregulin 1
Synonyms ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, NRG1, isoform Alpha, NRG1A, Pro NRG1, SMDF
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform Alpha.

实验方案

Product Specific Protocols
WB protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
IF protocol for NRG1, isoform Alpha antibody 31043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols