验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, human testis tissue, human placenta tissue, mouse brain tissue, HeLa cells, A549 cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 2 publications below |
产品信息
21549-1-AP targets NRBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | NRBP2 fusion protein Ag16215 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nuclear receptor binding protein 2 |
别名 | NRBP2, pp9320, TRG 16, TRG16 |
计算分子量 | 501 aa, 58 kDa |
观测分子量 | 33 kDa, 58 kDa |
GenBank蛋白编号 | BC113873 |
基因名称 | NRBP2 |
Gene ID (NCBI) | 340371 |
RRID | AB_10733219 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NSY0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.
实验方案
Product Specific Protocols | |
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WB protocol for NRBP2 antibody 21549-1-AP | Download protocol |
IHC protocol for NRBP2 antibody 21549-1-AP | Download protocol |
IP protocol for NRBP2 antibody 21549-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.
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Ann Transl Med PSMD12 promotes glioma progression by upregulating the expression of Nrf2. | ||
Mol Cell Biochem TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.
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Cancers (Basel) Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression. |