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NRBP2 Polyclonal antibody

NRBP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 21549-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

NRBP2, pp9320, TRG 16, TRG16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, human testis tissue, human placenta tissue, mouse brain tissue, HeLa cells, A549 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21549-1-AP targets NRBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 NRBP2 fusion protein Ag16215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor binding protein 2
别名 NRBP2, pp9320, TRG 16, TRG16
计算分子量 501 aa, 58 kDa
观测分子量 33 kDa, 58 kDa
GenBank蛋白编号BC113873
基因名称 NRBP2
Gene ID (NCBI) 340371
RRIDAB_10733219
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSY0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for NRBP2 antibody 21549-1-APDownload protocol
IHC protocol for NRBP2 antibody 21549-1-APDownload protocol
IP protocol for NRBP2 antibody 21549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.

Authors - Zhiyu Li
  • KD Validated
humanWB

Ann Transl Med

PSMD12 promotes glioma progression by upregulating the expression of Nrf2.

Authors - Zhongyong Wang
human,mouseWB,IHC

Mol Cell Biochem

TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.

Authors - Feng Chi
  • KD Validated
humanWB

Cancers (Basel)

Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression.

Authors - Anqi Xiong
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