验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human testis tissue, human placenta tissue, mouse brain tissue, HeLa cells, A549 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
21549-1-AP targets NRBP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16215 Product name: Recombinant human NRBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 138-258 aa of BC113873 Sequence: PLMNFAATRPLGLPRVLAPPPEEVQKAKTPTPEPFDSETRKVIQMQCNLERSEDKARWHLTLLLVLEDRLHRQLTYDLLPTDSAQDLASELVHYGFLHEDDRMKLAAFLESTFLKYRGTQA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor binding protein 2 |
| 别名 | NRBP2, pp9320, TRG 16, TRG16 |
| 计算分子量 | 501 aa, 58 kDa |
| 观测分子量 | 33 kDa, 58 kDa |
| GenBank蛋白编号 | BC113873 |
| 基因名称 | NRBP2 |
| Gene ID (NCBI) | 340371 |
| RRID | AB_10733219 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSY0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NRBP2 antibody 21549-1-AP | Download protocol |
| IP protocol for NRBP2 antibody 21549-1-AP | Download protocol |
| WB protocol for NRBP2 antibody 21549-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.
| ||
Ann Transl Med PSMD12 promotes glioma progression by upregulating the expression of Nrf2. | ||
Mol Cell Biochem TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.
| ||
Cancers (Basel) Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression. |
