NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

HRAS1, NRAS, EC:3.6.5.2, GTPase NRas, N ras

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA431 cells, Recombinant protein, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NRAS Ready-To-Use IHC Kit. NRAS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma RAS viral (v-ras) oncogene homolog
别名 HRAS1, NRAS, EC:3.6.5.2, GTPase NRas, N ras
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC005219
基因名称 NRAS
Gene ID (NCBI) 4893
RRIDAB_2918061
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01111
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.

实验方案

Product Specific Protocols
WB protocol for NRAS-Specific antibody 18296-1-APDownload protocol
IHC protocol for NRAS-Specific antibody 18296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery and Characterization of RP03707: A Highly Potent and Selective KRASG12D PROTAC

Authors - Xiang Ji
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