验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, Recombinant protein, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NRAS Ready-To-Use IHC Kit. NRAS primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neuroblastoma RAS viral (v-ras) oncogene homolog |
| 别名 | HRAS1, NRAS, EC:3.6.5.2, GTPase NRas, N ras |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC005219 |
| 基因名称 | NRAS |
| Gene ID (NCBI) | 4893 |
| RRID | AB_2918061 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01111 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NRAS-Specific antibody 18296-1-AP | Download protocol |
| WB protocol for NRAS-Specific antibody 18296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Chem Discovery and Characterization of RP03707: A Highly Potent and Selective KRASG12D PROTAC | ||
Sci Signal Wild-type RAS signaling is an essential therapeutic target in RAS-mutated cancers.
| ||
J Med Chem Discovery and Characterization of RP04340: A Highly Potent and Orally Active Pan-KRAS PROTAC Degrader. | ||
Sci Signal HRAS promotes mutant NRAS-driven transformation with codon and allele specificity. |
