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验证数据展示

  • WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

    WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

  • WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

    WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

    IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

    IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

NRAP Polyclonal antibody, PBS Only

NRAP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25977-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25977-1-PBS targets NRAP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23254

Product name: Recombinant human NRAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1309-1393 aa of BC126407

Sequence: RHDFVKERGKLIGPQSVRDDPRIQHCRRMGQLQSELQYRRGATSSQAQFHLPMDMVHLVHAKNAQALASDHDYRTQYHKFTALPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nebulin-related anchoring protein
别名
观测分子量190-200 kDa
GenBank蛋白编号BC126407
基因名称 NRAP
Gene ID (NCBI) 4892
RRIDAB_2918098
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VF7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRAP (Nebulin-related-anchoring protein) is a striated muscle-specific protein that is concentrated at intercalated disks in cardiac muscle and at myotendinous junctions in skeletal muscle. NRAP is a 197 kDa multidomain scaffolding protein from nebulin family with many interation partners such as α-actinin, actin, vinculin and filamin (PMID:21276443). During cardiomyocyte development in the foetal heart, NRAP is involved in myofibrillar assembly and links terminal actin filaments to membrane complexes in the adult heart. Several studies observed that NRAP mutation would contributed to the dilated cardiomyopathy which is a highly heterogeneous disease with almost 100 candidates genes to date (PMID:28611399).

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